Starch "enzyme preparation" liquefaction auxiliary materials use these 3 methods-beer production

Eight craftsman Ding pine nuts remind everyone, the atmosphere turns cool, pay attention to adding clothes... Then we enter today’s theme:

According to the effect of actual production and the function characteristics of amylase preparations, there are three methods:

1, mixed heating Method:

is added at the same time when the auxiliary materials are fed, so that the amylase can be fully dispersed and freed in the mash. With the increase of the temperature of the auxiliary materials, the action speed of the enzyme also increases until the temperature of the mash gradually reaches and exceeds the loss of the enzyme. Live temperature until the enzyme stops working.

This addition is very convenient and the liquefaction effect is still good, but the following problems should be noted:

, auxiliary materials must be crushed relatively finely, in order to quickly gelatinize and liquefy, otherwise it will cause "peeling and gelatinization" (ie Large starch granules gradually gelatinize and liquefy inward layer by layer), so that after enzyme inactivation, the starch has not been completely liquefied, so that the mash is still thick;

, a variety of similar excipients with a lower gelatinization temperature should be used. Because of the high gelatinization temperature of auxiliary materials, the critical temperature of starch gelatinization will also inactivate or passivate some enzymes. In addition, the material water ratio of gelatinization mash is generally larger, and the heat resistance of amylase preparations will be somewhat In this way, when the gelatinization of the auxiliary materials at the gelatinization temperature is proceeding rapidly, the enzyme has gradually inactivated, which affects the liquefaction effect;

, when using the mixed lift method, you can consider adding some calcium ions (gypsum Or calcium chloride) to protect the effect of enzyme preparations. It is also possible to consider pre-soaking the auxiliary material to make the auxiliary material starch fully absorb water and swell and improve the effect of the enzyme.

2, intermediate input method:

first mix the auxiliary powder and water evenly and then put it into the gelatinization pot. It does not matter if the feeding temperature is slightly higher (for example, about 60℃), and then increase the temperature according to the specified heating rate. When the mash temperature is close to When the temperature of the amylase preparation is suitable, adjust the enzyme preparation with water into a uniform slurry solution and slowly pour it into the gelatinized mash (liquid enzyme preparation can be poured directly).

increases the temperature instantly when the enzyme solution is in contact with the gelatinized mash, so that the action speed of the enzyme is rapidly accelerated, that is, the swollen and gelatinized starch colloid solution can be quickly liquefied. This method is more troublesome for

. In addition, the gelatinized mash is thicker and the enzyme dispersion is poor.

In addition, if the bacterial amylase is a powder, it is easy to cause the bacterial spores in the enzyme preparation to fly away during the mixing, and the effect of this method is acceptable.

Use this method to pay attention to the following issues:

, the material-to-water ratio is required to be larger (5:1 or more), so as to avoid the mash is too thick after gelatinization into starch glue solution, which affects the dispersion of enzymes, resulting in poor effect Z3z

, relative to the mixing and heating method, this method is slightly less affected by the gelatinization temperature and crushing degree of the auxiliary materials, but it must be stirred to prevent the mash from sticking;

, the amount of enzyme may be slightly higher, the most Haoneng is calculated based on the use of 7~8u per 1g of auxiliary materials in liquefaction. Of course, it is better if 5~6u can meet the requirements of liquefaction.

, the heating speed should not be too fast to ensure that the auxiliary starch can fully absorb water and swell and rupture to meet the requirements of gelatinization.

3, instantaneous action method:

this method can be in two forms.

First heat the auxiliary material mash with about 2/3 of the water in the gelatinization pot, and then mix the auxiliary material powder and the enzyme preparation with the remaining water (about 1/3) in the other pot and mix them evenly. Mix, when the water temperature in the gelatinization pot reaches 90~95℃, use a pump to pump the enzyme and auxiliary material mixed slurry into the incubation pot in a fan-shaped dispersion, so that the auxiliary material powder can quickly absorb water and expand and gelatinize at the moment of contact with high-temperature water At the same time, it is quickly liquefied by enzyme action. Because the auxiliary materials are pre-soaked during the mixing process, the effect of gelatinization and liquefaction can be improved.

Another method is that the form of the slurry is unchanged, but the water volume can be adjusted to the water volume of the specified material-to-water ratio, and then the slurry is pumped into a special high-pressure nozzle, and high temperature is passed into the nozzle. The steam quickly heats up to instant gelatinization and liquefaction. The two forms of

have short action time and good effect, but they must meet the following conditions:

, auxiliary powder must be crushed very finely, and the fine powder of 60 mesh or 80 mesh should be no less than 80%, so that it can be in contact At the moment of heating up, it will quickly absorb water to expand, gelatinize and liquefy, otherwise the effect will not be good, and it will easily block the nozzle when using the second form;Prepare a slurry tank, and equip agitator, sprinkler and pump;

, use high temperature steam should pay attention to control its purity, or treat it appropriately, nozzle, slurry pipe, steam pipe should use stainless steel.

The above three methods can be selected according to conditions and effects for factories using bacterial amylase preparations.

However, in order to improve the effect of liquefaction, when using the mixed heating method and the intermediate input method, you can add 8~10g auxiliary powder per g of malt powder liquefaction. Put an appropriate amount of malt powder into the gelatinization pot first, and pay attention Keep the temperature at 70°C for 10-15 minutes, as a means to liquefy the auxiliary material to ensure the liquefaction of the auxiliary material mash.

In this case, the amount of bacterial amylase can be appropriately reduced (calculated based on 1g of auxiliary powder with enzyme 3~4u). If you can use high temperature resistant amylase, you can use any of the above methods, and the requirements are not very strict.