Sisters who have done in vitro fertilization should be familiar with "cyst-raising", which can further screen high-quality embryos with development potential by extending the in vitro culture time, thereby improving the clinical pregnancy rate. With the joint efforts of all the m

Sisters who have done test tube should have some understanding of "nurturing cysts". "Nursing cysts" can further screen high-quality embryos with development potential by extending the in vitro culture time, thereby improving the clinical pregnancy rate.

With the joint efforts of all the medical staff and laboratory technicians of Jiahui Reproductive Medicine Center, our clinical pregnancy rate of D3 embryos is 50%, and the clinical pregnancy rate of blastocysts has reached 59.6%.

Breeding cyst & blastocyst transfer

After the eggs and sperm are retrieved, insemination (IVF or ICSI) is performed in the embryo laboratory. The normally fertilized eggs are called fertilized eggs. The fertilized egg continues to develop until it reaches an 8-10 cell state on the 3rd day, which is called a D3 cleavage embryo. If the culture continues for 2-3 days, the embryo will develop into a blastocyst. The process of cultivating D3 cleavage embryos to blastocysts is called blastocyst culture , or "cultivating the blastocyst" for short. The process of transplanting an embryo that has developed to the blastocyst stage into the mother's uterus is called "blastocyst transfer."

Generally, under the same physical conditions, the greater the success rate of "nurturing the bladder", which means the higher the success rate of in vitro fertilization. Currently, the blastocyst formation rate of D3 cleavage embryos at Jiahui Reproductive Medical Center has reached 75%.

Figure: Dynamic development process of D1-D5 embryos

Blastocyst culture requires a continuous and stable culture environment. It not only includes a culture microenvironment with stable parameters such as temperature, osmotic pressure , and pH value, but also includes a stable operating incubator environment and a large laboratory environment equipped with a high-efficiency laminar flow filtration system (LifeAire).

PART.1 Advanced facilities facilitate high-quality blastocyst culture

Friends who have undergone egg retrieval surgery know that 3-5 days after the eggs retrieved on the day of the surgery are sent to the embryo laboratory, they will be notified that how many eggs have been grown during this cycle. embryo. The embryonic laboratory, as the heart of the reproductive center, has always been a mystery in the eyes of many people. What kind of place can turn an egg into an embryo? What are the hidden skills that are not known to anyone?

First of all, the embryo laboratory is a place with particularly high environmental requirements. The temperature and humidity here remain constant throughout the year. The air entering here needs to pass through the five layers of purification and filtration of the laminar flow system and reach the 10,000-level cleanliness level. The embryonic laboratory of Jiahui International Hospital has introduced the internationally advanced LifeAire laminar flow purification system . The indoor air has reached a thousand-level cleanliness level, fully ensuring the effective filtration of organic compounds, harmful particles and other air components in the laboratory. , further reducing the impact of ambient air quality on embryonic development and pregnancy outcomes.

Jiahui Embryology Laboratory LifeAire laminar flow purification system

Jiahui Embryology Laboratory uses a specially customized yellow light illumination system (yellow light wavelength is 580~595nm), which can effectively avoid wavelengths that have an impact on embryonic development (<>

Reference values ​​of wavelengths of different colors of light

Secondly, Jiahui Embryo Laboratory is equipped with a new generation of time difference incubator (Embryo scope plus). During the embryo culture process, the embryos can be observed at any time without taking them out from the incubator, and the embryo development can be seen. the complete process. can obtain complete information about embryonic development without any interference to the embryonic development process, which can help clinicians choose transplantation and freezing strategies for this cycle and make good use of every embryo. Observing the culture process of thawed embryos through the time difference incubator can also understand the recovery process of embryos after thawing and predict the developmental potential of embryos.

Jiahui embryo laboratory jet lag incubator

PART.2 Strict quality control to ensure high-level culture results

In order to provide a high-quality culture environment for each embryo, the embryologists in the embryo laboratory closely monitor every influence that may affect the embryonic development process. factor.Carefully carry out quality control of each batch of reagents and consumables, and monitor the operating status of incubators, microscopes, ultra-clean workbench and other equipment on a daily basis. Regularly detect air particles, VOC ( volatile organic compounds ), air culture, etc. in the embryo laboratory to ensure the continued stability of the laboratory environment. The quality control work in the embryo laboratory is fragmented and tedious, but it is very important and requires the embryologist to be extremely patient and careful. It is the embryologists in the embryology laboratory who carefully and carefully conduct quality control day after day, which lays the foundation for the high blastocyst formation rate of Jiahui Reproductive Medicine Center.

PART.3 Strive for excellence and care for every embryo

The embryologists in the embryology laboratory of Jiahui Reproductive Medicine Center are not only the technical backbone of domestic tertiary hospitals and private hospitals, but also have well-known foreign experience in the United States, the United Kingdom, Japan, Canada, etc. Fertility center work experience. Such an experienced team holds regular exchange meetings to continuously optimize and integrate many complex laboratory operating steps such as gamete processing, in vitro fertilization, embryo culture, etc., abandon unnecessary habitual operating procedures, and simplify routine operating procedures. The integrated operation process makes the entire operation procedure simple, fast and smooth, reducing interference to the embryos and reducing the time the embryos are exposed outside the incubator.

Jiahui Reproductive Medicine Center adheres to the principle of single embryo freezing, that is, each freezing carrier only carries one embryo. The embryologist only freezes one embryo per operation, and based on the shrinkage and expansion of each embryo, within the time range recommended by the cryoprotectant manufacturer, individually determines the specific equilibrium time of each embryo in the cryoprotectant. , try to tap the frozen recovery potential of each embryo as much as possible, Currently, the thawing recovery rate of our frozen blastocysts is 100%.

· Summary ·

Due to the above advantages of blastocyst transfer, we generally recommend that everyone give priority to blastocyst transfer when conditions are suitable. However, for sisters who have experienced failed engraftment, don’t be too anxious, because our doctors will customize the embryo transfer and transplantation according to the actual situation of each patient. After analyzing and weighing the pros and cons of various options, Freeze or continue culture to make the most logical choice. We believe and wish that every family can have a healthy baby!

Contributor and reviewer: Jiahui Medical Reproductive Medicine Center

Editor: Wan Jie

Source: Pexels