PCR is the abbreviation of polymerase chain reaction (polymerase chain reaction, PCR), and is a method for enzymatic synthesis of specific DNA fragments in vitro. It consists of reactions such as high-temperature denaturation, low-temperature annealing, appropriate temperature extension, etc., and the cycle is carried out to rapidly amplify the target DNA. It has the characteristics of strong specificity, high sensitivity, simple operation and time-saving. PCR certificate is the certificate of clinical gene amplification technicians. Compared with nurse certificate , it is less difficult to get the exam, but it is a necessity in -related positions in nucleic acid testing, and there will also be senior junior high school professional titles. Under the current normalized emergency response mechanism, since the professional title requirements are difficult to meet, you only need to be certified to take the post.
Work tasks
1. Responsible for the storage, storage and delivery of samples;
2. Extract and purify ribonucleic acid or DNA ;
3. Detection of extracted nucleic acids through real-time fluorescence quantitative polymerase chain reaction;
4. Construct the library and check and identify the quality of the library according to the sequencing standards;
In fact, the release of a nucleic acid report requires many rigorous steps, involving many links of the staff. The entire process of nucleic acid testing for new coronavirus pneumonia includes sample collection, standard transmission, sample reception and entry, reagent preparation, sample addition and extraction, computer amplification, result review and data transmission. Sampling is just a step in nucleic acid detection, but the nucleic acid sampler is the front end of the entire nucleic acid detection. Usually a small nucleic acid sampling point requires multiple staff members, including sampling, documents, transportation, disinfection, security, etc. In contrast, nucleic acid samplers have professional thresholds.
