Da Wan News The reporter learned from of the University of Science and Technology of China that recently, Professor Zhao Gang from the school of Information Science and Technology of , and Professor Shi Qinghua from the School of School of Life Sciences, and Professor Cao Yunxia from the Reproductive Medicine Center of the First Affiliated Hospital of Anhui Medical University. Based on the synergistic ice suppression effect of structural bionics and space physics, he successfully achieved high-quality deep and low temperature preservation of mouse follicles . Related research results were recently published in the international journal Nature Communications. This research result is of great reference value for follicle-based preservation of female fertility.
fertility preservation is the only way to solve the major health problem of reducing female fertility, a common concern for all mankind. Because of the rich source of follicles, easy access, and no excessive ethical issues involved, its cryopreservation is an important supplement to the cryopreservation of oocytes, , embryos and ovarian tissues, etc.; in addition, follicles preservation of minor women and unmarried female tumor patients is almost the only means of preservation of fertility. The existing follicle low-temperature storage methods have caused excessive concentration and excessive toxicity due to the excessive follicle concentration of the low-temperature protective agent used.
Figure. Synergistic ice-inhibiting and low-temperature preservation of mouse follicles and 3D culture in vitro maturation
Based on this, the low-temperature biomedical research team of the University of Science and Technology of China developed a vitrification preservation method for low-concentration low-temperature protector of mouse follicles based on a synergistic ice-inhibiting strategy. They combined magnetothermal and photothermal spatial retemperature with hydrogel microencapsulation to achieve synergistic ice suppression, reducing the concentration of permeable protective agent by 75% (only about 1/4 of the original). Among them, spatial re-temperature technology can effectively improve the re-temperature rate and temperature distribution uniformity, inhibit the possible anti-vitrification/recrystallization of the re-temperature process, and avoid excessive cell damage. hydrogel encapsulation can not only provide key biochemical and mechanical microenvironment regulation, but also effectively support the 3D culture and development of mouse follicles after re-temperature; it can also provide structural bionic low-temperature protection to reduce the need for toxic and side-induced low-temperature protectors; it can also achieve physical isolation between follicles and external magnetothermal and photothermal materials, improving biosafety.
The survival rate of follicles preserved by this method increases by about 1/3 after frozen resuscitation; and after in vitro 3D culture, mature oocytes can be successfully discharged, which are further fertilized in vitro and transplanted into surrogate mice and can give birth to healthy progeny mice. To sum up, the study established an integrated platform that integrates microencapsulation, freezing, regenerative and 3D culture, providing a unique solution for the preservation and full utilization of follicles.
Dawan News reporter Chen Mu
(Anhui.com)
