[Reprint] Nano Res. │ General strategy for glucose detection based on metal-organic framework nanoenzyme probes* Today's Toutiao has limited format. For a better reading experience, please go to the WeChat official account. *Go to the official account of "Nanozymes" to learn the

[Reprint] Nano Res. │ General strategy for glucose detection based on metal-organic framework nanoenzyme probes

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*This article was first published on the "Nanozymes" public account, September 29, 2022

*Editor: Gu Xiang

The following articles are from NanoResearch, author Jiang Guohua

NanoResearch.

Nano Research is a peer-reviewed international research journal published by Tsinghua University Press & Springer.

Reprint Editor: Yu Jiyuan

Background introduction

Glucose mg in many complex substrates such as biology and food and beverages. Highly sensitive detection of glucose in various complex substrates can provide effective reference guidance for food processing and health management needs, and has important application value in many fields such as biomedical , food safety, analytical chemistry , etc. However, in many matrix samples with complex compositions, such as colored liquids or body fluids, interfering impurities such as pigment molecules, biological macromolecules, etc. will affect the accuracy of conventional testing solutions. In addition, many commercial glucose kits use peroxidase as a catalyst, which has limitations of chemical stability and high preparation cost. Therefore, how to eliminate impurity interference and avoid cumbersome pretreatment processes and achieve sensitive and cost-effective glucose molecular detection in complex substrates remains an important challenge.

Introduction to the results

Southeast University Cui Yiping/Wang Zhuyuan's team proposed a highly sensitive glucose detection solution suitable for complex matrix environments. This scheme uses MBs@MIL-100(Fe)@Ag(MMA) with excellent SERS activity and peroxidase-like catalytic activity as the SERS probe, and uses the TMB molecule that can be detected specifically by MMA as the Raman beacon to achieve high sensitivity SERS detection on glucose through a catalytic cascade reaction between MMA and glucose oxidase. Based on the enrichment and size screening function of the metal-organic framework nanoenzyme MIL-100 (Fe), this strategy has excellent anti-interference characteristics. Even in colored beverages or complex biological fluids (saliva), MMA can still specifically enrich and detect indicator molecules and eliminate the influence of other impurities (dyes, proteins, etc.). In addition, MMA's excellent catalytic self-cleaning performance can also enable circular multiplexing detection of probes, reducing the detection cost of this solution, and providing a new general strategy for accurate glucose detection of complex matrix samples such as biological fluids and food and beverages.

Graphics and text introduction

Figure 1 (a) Preparation process of MMA probe. (b) Schematic diagram of the specificity and cyclable detection principle of glucose molecules in complex substrates.

Figure 2 (a) VSM diagram of the MMA probe. The digital photo in the figure shows the rapid magnetic separation process of MMA scattered in deionized water. (b) extinction spectrum of different catalytic systems, the digital photo on the upper right is the real picture corresponding to different catalytic systems. (c) SERS spectrum of different concentrations of CV were detected by MMA. (d) Calibration curve of CV concentration and corresponding SERS intensity at 1172 cm−1 peak position in Figure c.

Figure 3 (a) Comparison of SERS detection results of MMA in water, 10% BSA and mixed dye solution (sunset yellow, methyl orange , erythriller , amaranth red ). (b) MMA cycle to detect glucose in purple beverages. (c) MMA cycle to detect glucose in saliva. (d) Comparison of commercial GOD-POD glucose kits with this protocol to monitor changes in saliva glucose content after eating large amounts of sugar for 1 h.

Author Profile

Professor Cui Yiping and Professor Wang Zhuyuan of the Advanced Photonics Center of the School of Electronic Science and Engineering, Southeast University are the joint corresponding authors of the article, and the research group doctoral student Jiang Guohua is the first author of the article.

Cui Yiping , professor, doctoral supervisor, National Outstanding Youth Science Foundation winner, and member of the American Optical Society (OSA Fellow). He has successively presided over and completed dozens of national and provincial and ministerial research projects such as key projects of the National Natural Science Foundation, 973 projects, and 863 projects. More than 400 high-level papers have been published in domestic and foreign academic journals such as J. Am. Chem. Soc., Chem. Rev., Advanced Materials, ACS Nano., and more than 30 invention patents have been authorized, and he cited more than 10,000 articles. He participated in the compilation of works such as "Laser Physics", "Strong Light Optics and Its Applications", and "Physics of Nonlinear Optics".

Wang Zhuyuan , professor, doctoral supervisor, young chief professor of Southeast University, winner of the National Outstanding Youth Science Foundation, winner of the Jiangsu Province Outstanding Youth Science Foundation, and the high-level talent training target of Jiangsu Province's "Six Talent Peaks". He was selected as the Jiangsu Province 333 High-level Talent Training Plan. It has successively undertaken national and provincial and ministerial scientific research projects such as the National Natural Science Foundation Youth Project, Jiangsu Outstanding Youth Fund, and 973 Project. He has published more than 170 academic papers included in SCI in international journals such as Chem. Rev., J. Am. Chem. Soc., ACS Nano, and he cited more than 4,400 articles and has more than 20 authorized invention patents.

Article information

G. Jiang, Z. Yang, K. Zhu, et al. Universal peroxidase-like strategy for sensitive glucose detection in complex matrix. Nano Research. https://doi.org/10.1007/s12274-022-4788-2.