3. New coronavirus nucleic acid testing laboratory design parameters (for reference) Generally, PCR laboratories do not have strict purification requirements, so it is necessary to determine the cleanliness of the laboratory based on the experimental content. When PCR experimenta

3. New coronavirus nucleic acid testing laboratory design parameters (for reference)

Generally, PCR laboratories do not have strict purification requirements, so it is necessary to determine the cleanliness of the laboratory based on the experimental content. When PCR experimental samples need to be protected, the laboratory should be equipped with a clean air conditioning system. For new coronavirus nucleic acid testing laboratories, it is recommended to set up 100,000-level purification.

6. Decoration of the new coronavirus nucleic acid testing laboratory
The nucleic acid testing laboratory has several key points such as design concept, decoration requirements, purification air conditioning system, ventilation, automatic control, and process equipment.

Material: partition wall and ceiling - purification color steel plate, floor - homogeneous core-transparent PVC floor, special door for purification laboratory, observation window;
requirements:
1. Wall surface: smooth, no dust accumulation, resistant Corrosion, easy to clean and disinfect, no gaps;
2. Floor: anti-static, corrosion-resistant, seamless, no leakage;
3. Yin and yang corners: sealed with arc corners;
4. Doors: automatic interlocking (mechanical) must be set or electronic);

purification air conditioning, ventilation system, automatic control system
1. Avoid cross-contamination and use a fresh air conditioning system;
2. The sample preparation area is designed and constructed in strict accordance with BSL-2;
3. The exhaust system all adopts high-efficiency filtration as required purification device;
4. Air flow organization: adopts the form of upper and lower rows;
5. Pressure gradient control is controlled by a micro-pressure differential meter and a precision automatic control system;
6. All index parameters in the room are displayed at the entrance of the laboratory, and a deviation alarm is set ;
process equipment
1. The lower part of the experimental bench is left empty, without dead ends;
2. All made of stainless steel 304;
3. Sample preparation area biological safety cabinet : BSC-||-B2 type;
4. The sample preparation area is equipped with eyewash Devices, emergency sprinkler devices when necessary;
5. Non-contact operation concept (sensor faucet, sensor automatic door, interlocking transfer window, etc.);

Laboratory construction involves material selection, stability, precision control, etc. Multiple technical and knowledge areas. Different laboratories have different requirements for laboratory construction due to different test samples and test methods. It can be said that there are many differences. A complete laboratory plays an important role in analysis and testing, and also plays an important role in ensuring the health of laboratory workers.

7. Laboratory equipment

New coronavirus testing should be carried out by qualified medical institutions (including independent medical testing laboratories) and disease control institutions. Medical institutions that carry out pathogenic testing must have laboratory qualifications of level 2 or above registered with the health authority of the people's government at the districted municipal level, and comply with the "Measures for the Management of Clinical Gene Amplification Testing Laboratories of Medical Institutions" ( According to the requirements of the Ministry of Health Medical Affairs (2010) No. 194), it has PCR laboratory conditions. Medical testing laboratories should comply with the requirements of the "Basic Standards for Medical Testing Laboratories (Trial)" and "Management Specifications for Medical Testing Laboratories (Trial)" (Guo Wei Yi Fa [2016] No. 37); medical institutions or disease control agencies will If the virus testing business is entrusted to a medical testing laboratory, a corresponding agreement shall be signed with it as required to clarify the rights and obligations of both parties.

From left to right, they are: reagent preparation area, sample preparation area, amplification area and product analysis area, a total of 4 areas area.
safety protection: ultra-clean workbench , biological safety cabinet, goggles, protective clothing, medical mask N95, disposable latex gloves
sample reagent storage: ultra-low temperature refrigerator , low temperature refrigerator, reagent storage cabinet
sample reagent processing : Nucleic acid extractor, constant temperature water bath, metal bath/dry bath, ultrapure water machine , palm/high-speed refrigerated centrifuge, mixer, analytical balance
sample nucleic acid detection: fluorescence quantitative PCR
reagents and consumables: Pipette , sterilized centrifuge tubes, pipette tips, nucleic acid extraction reagents/nucleic acid detection reagents, 96-well plates, etc.
disinfectant sterilization: air purification and disinfection machine , high pressure sterilizer