Title: Sustained Inhibition of Maize Seed-Borne Fusarium Using a Bacillus-Dominated Rhizospheric Stable Core Microbiota with Unique Cooperative Patterns.

Title: Sustained Inhibition of Maize Seed-Borne Fusarium Using a Bacillus-Dominated Rhizospheric Stable Core Microbiota with Unique Cooperative Patterns

Using the rhizosphere stable core microbiota with a unique cooperative model dominated by Bacillus to sustainably inhibit maize seed-borne Fusarium

Journal: Advanced Science

Corresponding author: Zhang Ruifu

Abstract

species of pathogenic bacteria can inhabit the rhizosphere and infect plants after germination. The rhizosphere microbial community plays a key role in resisting seed-borne pathogens. However, the mechanisms by which core rhizosphere microbiota are constructed to suppress seed-borne pathogens remain unclear. In a sterile environment, the root-associated microbial population was infected with seed-borne Fusarium species, but the root-associated microbiota was not infected when interacting with the local soil microbiota, indicating that the core rhizosphere microbial community was constructed to inhibit seed-borne Fusarium species. Core rhizosphere bacteria were identified using two strategies: stepwise dilution and rhizosphere deposition attraction. A synthetic bacterial consortium (SynM) was constructed using core rhizosphere bacterial isolates and optimized based on superior community stability and antibacterial ability. The synthetic bacterial consortium was superior to single strains and randomly formed microbial communities. Optimized SynM (OptSynM) proposes a unique cooperation model in which the key strain inhibits Fusarium by synthesizing the -antagonizing substance fentanine, while other members function through the expression of related genes that promote growth, antagonize and promote plant growth. This study demonstrates an innovative approach to building a stable and minimal microbiota for sustainable agriculture and proposes a unique cooperative model to maintain community stability and functionality.

Main results images

Figure 1: Experimental design diagram.

Figure 2: Seed-borne Fusarium species in corn varieties and their distribution in non-sterile and sterile soils.

Figure 3: Core bacteria identified using stepwise dilution.

Figure 4: Using root exudates to attract and identify core bacteria.

Figure 5: Construction and simplification of synthetic bacterial flora.

Figure 6: Cooperation model of synthetic bacterial flora. Introduction to

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