Anyone who studies stem cells should know that human pluripotent stem cells (hPSCs) can acquire genetic and epigenetic changes during in vitro culture. Given the increasing use of hPSCs in research and therapy, and the substantial increase in the number of hPSC lines available to researchers, the International Society for Stem Cell Research has recognized the need to reevaluate the quality control standards to ensure the genetic integrity of hPSCs.
hPSCreg is a global hPSCs registry (https://hpscreg.eu/) that currently lists approximately 4000 different hPSCs lines in its curated database. Recently, relevant researchers published an article titled: "The consequences of recurrent genetic and epigenetic variants in human pluripotent stem cells", which summarizes the properties of genetic and epigenetic variants that are currently repeated in hPSC culture, detection methods, and known knowledge about their impact on cell behavior in vitro or in vivo, and evaluates the safety of hPSC-derived cell therapies.
Researchers summarize the current nature of recurrent genetic and epigenetic variants in hPSC cultures, detection methods, and what is known about their effects on cell behavior in vitro or in vivo. The researchers believe that the potential consequences of low-level contamination of cell therapy products with cells harboring cancer-causing variants are currently largely unknown and highlight key challenges facing the field, particularly involving the safety assessment of HPSC-derived cell therapies. The extensive research on
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