Significant advances in the newly designed SMRT Cell, computing and new system architecture will enable Rvio to significantly increase throughput and reduce sequencing costs while leveraging the power of HiFi for superior accuracy and direct methylation detection.

2025/06/2221:48:36 science 1877

Significant advances in the newly designed SMRT Cell, computing and new system architecture will enable Rvio to significantly increase throughput and reduce sequencing costs while leveraging the power of HiFi for superior accuracy and direct methylated detection.

Early Screen News: On October 25, 2022, PacBio (Nasdaq stock code: PACB) announced the launch of the Revio long-read long sequencing system , which will enable customers to significantly expand their use of PacBio's world-famous HiFi sequencing technology. Revio aims to provide customers with the ability to sequence up to 1,300 human whole genomes at 30 times coverage per year, and each HiFi human whole genome sequencing costs less than $1,000. With this throughput and pricing, PacBio believes that Revio will enable HiFi sequencing to be used in large-scale research in human genetics, cancer research, agricultural genomics and other aspects.

Christian Henry

PacBio President and CEO

"Our customers have changed genomics perceptions with HiFi sequencing. Revio will further unleash this power by increasing high throughput and affordability. We designed a brand new SMRT Cell with three times the density of our existing SMRT Cell 8M, which has 25 million ZMWs. Revio is able to parallel up to 4 SMRT Cells simultaneously, and it can provide up to 100 million ZMWs at the same time Perform single-molecule real-time sequencing. Combined with our significant advances in computing, Revio will provide shorter runtime and increase HiFi data throughput by 15 times. I look forward to seeing researchers making new discoveries with the power of Revio.”

Scientists have achieved many "firsts" through HiFi sequencing on PacBio's Sequel IIe system: The first complete telomeres to telomeres human genome assembly (Nurk 2022), the first methylation group of haplotype parsed in rare disease cohorts (Cheung 2022), the first population survey of long-read long-structure variants (All of Us Research Program), the first single-cell level complete catalog of transcript isomers (Al'Khafaji 2021) and the first complete assembly of highly complex oat genomes (European Seed 2020). The Revio system uses the same groundbreaking HiFi reagents – producing accurate raw long read lengths, uniform sequencing coverage, unparalleled variation detection accuracy and assembly integrity, and accurate DNA methylation assays, all at a larger throughput scale.
Revio will be the first system of PacBio to use the NVIDIA GPU of , and compared with Sequel IIe, Revio's computing power is 20 times higher. In addition to providing accelerated basecalling to meet the higher throughput of Revio, the calculations supporting AI will also integrate deep learning algorithms to detect DNA methylation in the standard sequencing library, as well as DeepConsensus, a deep learning method developed with Google Health to improve HiFi production and sequencing accuracy.
Revio system reduces consumable usage by half and makes significant improvements in workflow and convenience compared to the Sequel IIe system. Revio can set up subsequent samples runs at the moment of the sample sequencing run, which provides operators with greater scheduling flexibility to load runs at any time of the day without causing consumable-related instrument downtime.

Significant advances in the newly designed SMRT Cell, computing and new system architecture will enable Rvio to significantly increase throughput and reduce sequencing costs while leveraging the power of HiFi for superior accuracy and direct methylation detection. - DayDayNews

Tomi Pastinen MD

Director of Genome Medicine Center, Kansas City Children's Charity Center

"In our Children's Genome Answers (GA4K) program, HiFi genome sequencing shows real advances beyond contemporary genetic analysis in unresolved rare disease samples. Improving the throughput of the Revio system at a lower cost will speed up sample answers to the GA4K project."

Gina Zastrow-Hayes Dr.

Corteva Agriscience Genomics Technology Manager

" The new Revio sequencing system from PacBio will become a key component of the Corteva Genomics Toolbox. Long read and long sequencing enables the identification of characterization of complex plant genomes, and now Revio’s high-throughput capabilities will allow us to apply HiFi technology more widely to agricultural biological applications. ”

Revio’s US catalog price is $779,000. PacBio is now accepting orders and is expected to start delivery in the first quarter of 2023.

PacBio also posted a presentation on its website with more details about Revio. Interested people can read the presentation on PacBio’s investor relations website, as well as product information.

About PacBio

Pacific Biosciences of California, Inc. (NASDAQ: PACB) is a leading life sciences technology company dedicated to designing, developing and manufacturing advanced sequencing solutions to help scientists and clinical researchers solve genetic complexity. The products and technologies we are developing are derived from two highly differentiated core technologies focusing on accuracy, quality and integrity, including our existing HiFi long-read sequencing and our emerging SBB™ short-read long sequencing technology. Our products address a wide range of research applications including human germ cell sequencing, plant and animal science, infectious diseases and microbiology , oncology, and other emerging applications. For more information, visit www.pacb.com.

PacBio Products are for research purposes only. Not used for clinical diagnosis.