Researchers such as Edward Lemke of the European Molecular Biology Laboratory (EMBL) took advantage of the characteristics of liquid-liquid phase separation to enrich specific proteins, inducing phase separation on the surfaces of different membrane structures and enriching the p

European Molecular Biology Laboratory (European Molecular Biology Laboratory, EMBL) Edward Lemke and other researchers used the characteristics of liquid-liquid phase separation to enrich specific proteins, inducing phase separation on the surfaces of different membrane structures and enriching orthogonal reaction sites. The required components achieve the effect of adding different unnatural amino acids to the two protein localizations in the same eukaryotic cell at the same time.

Researchers believe that this technology can greatly expand the space for protein manipulation.

This work was published in Cell(1) on August 24, 2021.

Comments:

very cleverly trades space for multiple channels, and there is room for further expansion; what needs to be noted is the impact of this phase separation system on normal cell membrane function, and the decrease in the specificity of protein orthogonal synthesis caused by membrane fluidity.

https://www.cell.com/cell/fulltext/S0092-8674(21)00943-0

Corresponding author introduction:

https://lemkelab.com/the-team/about-edward-lemke/

References:

1 . C. D. Reinkemeier, E. A. Lemke, Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation. Cell, 1–18 (2021).

Original link:

https://www.cell.com/cell/fulltext/S0092-8674 (21)00943-0

Note : Originally published on the public account "CNS Guide"