*Superscripts and subscripts cannot be displayed on Toutiao today. For a better reading experience, please go to the WeChat official account. On July 24, 2021, the Nanozyme Sub-forum of the 19th China Biophysics Congress was successfully held in Hefei.

*Superscripts and subscripts cannot be displayed on Toutiao today. For a better reading experience, please go to the WeChat official account.

*Go to the " nanozymes Nanozymes" public account to learn the latest news about nanozymes!

*This article was first published on the "Nanozymes" public account on September 24, 2021

*Editor: Yu Jiyuan

On July 24, 2021, the 19th China Biophysics Conference·Nanozymes Sub-Forum was successfully held in Hefei. The theme of this forum is "Nanozymes: Opportunities and Challenges of the New Generation of Mimic Enzyme ".

nanozymes are functional nanomaterials containing enzymatic properties. Since the research team of Chinese scientist Yan Xiyun reported the first ferric oxide nanozyme in 2007, the field of nanozyme research has developed vigorously, forming a new field of nanozyme research.

The forum is chaired by Chinese Academy of Sciences academician, Institute of Biophysics, researcher Yan Xiyun, and Chinese Academy of Sciences Changchun Institute of Applied Chemistry researcher Qu Xiaogang. Academician Yan Xiyun, Professor Zhou Ruhong, Professor Liu Junqiu, Researcher Gao Lizeng, Researcher Jiang Xiu'e, Professor Zhang Xiaodong, etc. attended the meeting and gave invited reports.

Click the link at the end of the article to watch all the recorded videos of this sub-forum meeting.

Speaker introduction

Based on the relevant conference reports and academic exchanges of the first China Nanozyme Conference held in Changchun in May, Academician Yan Xiyun put forward new thinking on nanozyme research. At this conference, he gave a speech entitled "My thoughts on nanozymes" A report on "Some Thoughts on Nanozyme Research".

Based on the development history of nanozymes, Academician Yan Xiyun mainly focused on four aspects of new thinking on "Characteristics of Nanozymes", "Standardization of Nanozymes", "Natural Properties of Nanozymes", and "Mimicking Enzymes/New Enzymes?" was elaborated.

Academician Yan Xiyun summarized the characteristics of nanozymes as follows:

1. Original in China and developing rapidly, China's research level on nanozymes is far ahead of European and American countries in terms of the concept and the acquisition of results;

2. It has relevant catalytic characteristics, but is different from natural enzymes, chemical catalysis, traditional simulated enzymes, and nanocarrier-immobilized enzymes, and has unique advantages.

This report mainly introduces the work of Professor Zhou's team using molecular simulation and artificial intelligence methods to study the interaction between nanoparticles and proteins.

Based on technologies such as QM, QM/MM, MD, FEP and Continuum Modeling, Multiscale Modeling has experienced a process transformation from QM to MM to Continuum, and has outstanding achievements in glucose detection, neuron signal detection, electrode design, etc. application potential. The

report explains the specific method of using graphene oxide to regulate the activity of lipase , and uses pictures and animations to demonstrate the process of enzyme fixation and opening of specific structures, further explaining the mechanism of its performance changes at the molecular level. In addition, using PEG to modify graphene can achieve a higher level of immune response . Professor Zhou Ruhong further introduced the mechanism of action in detail from aspects such as cytokine , and discussed the biological safety of the system. .

Professor Liu mainly introduced the research on selenium mimetic enzymes in this report. His team has conducted various studies on using selenium nanomaterials to simulate GPx catalytic structures.

During the antioxidant process in the body, glutathione peroxidase (GPx) plays an important role in scavenging hydroperoxides. Its catalytic center contains selenocysteine ​​, and its structure is conducive to substrate interaction. Catalytic center binding. In the catalytic process of

enzyme, it needs to go through two steps: recognition and catalysis.Research on imitated enzymes can start with the collaboration of their recognition sites and catalytic sites to make breakthroughs. In terms of recognition steps, Professor Liu's team further improved the substrate selectivity of selenium mimetic enzymes by designing substrate recognition sites similar to those of natural enzymes. In terms of catalytic steps, molecular imprinting experiments are used to design catalytic sites that reduce the reaction activation energy. Proximity effects are used to identify substrates using specific enzymes, antibodies and other natural proteins that can recognize glutathione . The modified artificial selenase Reached natural GPx levels.

In this report, researcher Gao Lizeng mainly introduced the antibacterial properties of iron sulfide nanozymes and their bactericidal and synergistic enhancement mechanisms, and shared relevant examples of their application in the treatment of cholecystitis , enteritis , and burns.

In recent years, research on the antibacterial properties of nanozymes is booming, from the discovery of nanozyme antibacterial activity, to the design of intelligently responsive, multifunctional nanozyme material systems, to the development of new nanozyme materials for specific disease infection types. The research team used ferric oxide nanozyme to simulate natural immune enzymes to catalyze sterilization, use its peroxidase activity to clear oral biofilm, prevent and treat caries , inhibit intracellular salmonella, and use its oxidase activity to catalyze inactivation influenza virus and expanded to other lipid envelope viruses. Researchers found that iron sulfide nanozyme has unique antibacterial properties and revealed that its rapid bactericidal mechanism is the induction of bacterial ferroptosis and the synergistic enhancement of polysulfide, and used it to treat cholecystitis, enteritis, burns, etc.

This report mainly introduces several typical nanozymes that can overcome the adverse limitations of the tumor microenvironment and their related tumor diagnosis and treatment applications.

Cancer is currently one of the main causes of human death. The development of nanotechnology has brought new hope to tumor treatment. It can not only improve the treatment efficiency of radiotherapy , chemotherapy and other traditional therapies, but also develop a series of non-destructive, low-cost New treatment methods for poisoning (PDT, PTT, etc.). However, no matter what method is used, its therapeutic efficiency will be limited by the tumor microenvironment (characteristics of hypoxia, slightly acidic, high content of ROS and GSH), and nanozymes can regulate the redox metabolism of cells, thereby improving the efficiency of tumor diagnosis and treatment. .

This report mainly introduces the research of cluster enzymes by Professor Zhang’s team.

In nanozyme research, how to precisely control the scale of nanozymes is a major problem, which hinders the biological application of nanozymes. In order to solve this problem, Professor Zhang's team proposed the concept of cluster enzymes. The

cluster enzyme can precisely control the structure of atoms and , thereby quantitatively controlling the activity. To this end, we screened a large number of element clusters and selected a relatively simple and representative copper-cadmium cluster structure, and modified and designed the catalytic activity and catalytic selectivity to gradually improve its catalytic effect. The obtained cluster enzyme has good biological properties and has a good alleviating effect on neuroinflammation.

Editor: Yu Jiyuan

Video link: https://apph4h91iv01443.h5.xiaoeknow.com/v1/course/column/p_614ac876e4b0b558b932b4f0?type=3