Time: July 05, 2022 11:37 Source: College of Biomedical Engineering
News from this site (Reporter Wang Bo, Correspondent He Zixuan) The development of a convenient and efficient construction method for the 3D distribution of Aβ plaques in the whole brain is important for promoting Al Research on Alzheimer's disease (AD) is of great significance. In order to solve a series of problems faced by the existing whole-brain Aβ plaque imaging technology, such as long experimental process cycle and low detection efficiency, the team of Professor Zhu Mingqiang of the School of Biomedical Engineering of Hainan University and the team of Academician of the Chinese Academy of Sciences Luo Qingming proposed a method that can be used. A new method for rapid 3D imaging of whole-brain Aβ plaques.
Recently, relevant research results were published in the "Journal of Chemical Engineering" (IF=16.744) under the title "Water-soluble AIE probe for three-dimensional imaging of Aβ plaques in the whole brain". Wang Yalong, Zhang Jianping, School of Biomedical Engineering, Hainan University, and Wuhan Luo Ting of the National Research Center for Optoelectronics is the co-first author, and Luo Qingming and Zhu Mingqiang are the co-corresponding authors of . This project has received funding support from the Ministry of Science and Technology’s Major R&D Program and the National Natural Science Foundation.
It is understood that this study synthesized a type of AIE-type water-soluble rinse-free Aβ probe that has greater affinity for Aβ fibers than the commercial probe ThT, and has better targeting and specificity for Aβ, and used this in situ labeling The probe was combined with the fluorescence microscopy optical section tomography system (fMOST), and through the cycle process of in-situ labeling/imaging/layer-by-layer cutting, the 3D efficient construction of Aβ plaques in the whole brain of Tg mice was achieved. The imaging time of plaques is shortened from 1-2 weeks to 3-4 days, significantly improving the efficiency of whole-brain 3D imaging.
This research not only provides design ideas for rinse-free AIEgens that match the fMOST system, but is also expected to be applied to 3D fluorescence imaging of deep tissues or entire organs, or even primate brains, playing an important role in future whole-brain research. effect.
Figure 1 (A) Chemical structural formula of water-soluble AIEgens. (B) Schematic illustration of in situ labeling of Aβ plaques with water-soluble AIEgens.
Schematic representation of the 3D imaging strategy for in situ labeling/imaging/slicing of whole-brain Aβ plaques.
Related paper information: http://dx.doi.org/10.1016/j.cej.2022.136840
(Editor: Wang Yiqin)
