Recently, many friends have been queuing up for nucleic acid every day. I have been asking, "Why has my nucleic acid report not come out yet?" I feel that only by getting a negative nucleic acid report will you feel at ease. Many people think that nucleic acid testing is like blood routine
In fact, nucleic acid testing is not as simple as you think
Citizens sample at the designated location according to the announcement time
The first step everyone understands is what is often called "punching your throat", open your mouth, and it is completed by professional sampling personnel at a specific sampling point. If it starts on time from 7 am, it generally takes 4-5 hours to sample nucleic acids in the city and can only be completed until 12 noon. After sampling, the specimens are sent to a specific laboratory for testing
After sampling, the specimens will be placed in the virus preservation solution. During the nucleic acid test process, due to the large number of nucleic acid specimens, in order to save testing time, a corresponding number of specimens is often collected, and a special person will be in charge of the vehicle to transport it to the laboratory in full armed manner.
After signing the specimens, they will be opened and entered into the system one by one
specimens will come to the laboratory and the testers need to unpack the outer packaging of the double-layer sealed biosafety bag one by one. Each time they are removed, they must spray alcohol to disinfect, and they must also protect biosafety .
With a "drop", the information of the specimen will be entered into the system to connect to the health code . During this period, it is completely manual, not much faster than sampling. Especially in a large number of specimens, we must ensure that there are 0 mistakes and we cannot misunderstand it.
shake specimens Extract nucleic acid
specimen before adding samples, in order to "shake" the virus from throat swab , sufficient vibration is required. Release the nucleic acid of the virus and reveal its "true face". Generally, it includes cleavage, binding, washing and other processes, and requires several repetitions to extract nucleic acids. During this process, medical staff still had to unscrew the lids one by one and use the sample loading gun to extract the specimen for nucleic acid.
Configure reagents in a completely polluted environment
Detectors need to configure a large number of detection reagents and auxiliary materials according to the number of specimens, and then accurately distribute them into each small tube. The number of reagents is as many as the number of samples, and the number of EP test tubes is the workload of thousands of gun tips. This step is completely polluted, strictly and accurately operated.
Here comes the PCR method that everyone often hears about - polymerase chain reaction, which is a technology to amplify viral nucleic acids. Only by infinitely amplifying the viral nucleic acid can the virus be detected in the specimen. The nucleic acid extracted from
is easily contaminated and easily degraded, so you must be more cautious and cautious in the operation! At the same time, the extracted nucleic acid only needs to be added to the reagent system for amplification. 5ul is equivalent to one tenth of a drop of water. Only a little bit on the head can be seen with a very small spear head. And it also requires the operation process: fast! stable! allow!
on-machine detection. After the test of nucleic acid specimens is started, you cannot stop and add new specimens in the middle. You must wait until the amplification of the results of this batch before the amplification of the next batch of specimens can be performed.
Review results
Guanghan Each round of nucleic acid collection was about 600,000 people, and about 62,000 tubes of specimens were collected. All specimens were tested on the machine and preliminary results were obtained, and often it was early morning of the next day.
At this time, you may have entered a sweet dream, but the Guanghan City Epidemic Emergency Command and the nucleic acid testing laboratory are still brightly lit.
If the
If the
Only after experts have judged it can a new epidemic prevention and control announcement be issued to everyone. At this time, it is often 4 or 5 o'clock in the morning. After our city found a positive nucleic acid test for a person returning to Guanghan from the city on October 6, we successively found positive cases in the fourth round of nucleic acid tests for all members of the whole region on October 9 and the seventh round of nucleic acid tests for all members of the whole region on October 12. The Guanghan Epidemic Emergency Command announced the epidemic prevention and control notice to the public. It was in accordance with the above strict work flow to meet everyone, ensuring the accuracy of the information.
Now, it can be seen that a "small" nucleic acid test report is coming. It covers so many steps. It takes so much time for the epidemic. Currently, the testers are investing in nucleic acid tests to shorten the time for the results. Your anxiety is also a big thing they care about! So I hope everyone will be more patient and understand that this epidemic will eventually pass, so let us wait for the flowers to bloom together!
Source: WeChat Guanghan, Laboratory Medicine
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