Just as machines need to be repaired and replaced regularly, the human body also has a self-repair mechanism, but in addition to the decline in the protection ability of telomerase, gene mutations are inevitable. This phenomenon will continue to accumulate in the body as we age,

cells last? Understand the things that cell freezes in one article!

Our body is a huge precision instrument, and every organ is an important component, and cells are the most basic and important components.

Just as machines need to be repaired and replaced regularly, the human body also has a self-repair mechanism. However, in addition to the decrease in the protection ability of telomerase , gene mutations are inevitable. This phenomenon will continue to accumulate in the body as we age, and the self-regeneration ability of cells will also decline with age.

Nowadays, many people have made long-term plans for their health and choose a new way of personal health management - frozen immune cells. But there are many questions: How long can cells be frozen? What is the activity after cell resuscitation? When is the highest quality of frozen cells?

Today, let the editor count the brief history of cell freezing and development, and let you learn about the things about cell freezing and storage.

cell frozen storage technology first appeared in the prototype

01

1776 , Spallanzani first published a report on the impact of "cold" treatment on "cell" life activities.

02

6 mid-to-late 19th century , many early workers (Prevost, 1840; de Quatrefages, 1853; Mantegazza, 1866; Scheuk, 1870) repeatedly studied the impact of cryogenic treatment on sperm activity, and came to a conclusion similar to Spallanzani, that is, "cold cannot kill sperm."

03

1900 , scientists basically affirmed the fact that biological components can be stored at subzero temperatures.

04

In the 1950s, Luyet and other scholars discovered the damage effect of electrolyte concentration on stored cells. Their basic conclusion is that increasing electrolyte concentration is the main cause of damage to stored cells.

cell freezing technology continues to develop

05

60s, Rowe, the blood center in New York, USA, achieved low temperature preservation of red blood cells . In 1980, he checked the red blood cell resuscitation after 12 years of storage at the liquid nitrogen temperature, and found no biochemical and functional variations, thus demonstrating in practice that biomaterial can survive for a long time at low temperatures.

06

70s, Mazur et al. first proposed the two-factor hypothesis on freezing damage, namely ice crystal damage and solution damage hypothesis based on the low-temperature preservation experimental data of Chinese hamster tissue culture cells.

07

80s, science and engineering have entered the field of low-temperature preservation research, and the continuous update and application of low-temperature engineering theory and practical design principles, and the rapid development of low-temperature biology research.

Since the 20th century, human scientific research has entered the cellular level stage and has begun to preserve and treat organisms and biological materials as food raw materials at low temperatures. By the end of the 20th century, with the continuous advancement of scientific methods and the continuous improvement of freezing methods, the low-temperature preservation technology was widely used in clinical practice.

cell storage

1. How long can cells be frozen?

can theoretically be stored permanently. studies show that cells can be stored in a refrigerator for a year in a -70℃ freezing, and cells can be stored in liquid nitrogen at a temperature of -196℃. As long as they are stored properly, the storage time is theoretically unlimited.

2, sleep for 15 years, and activity after recovery is 94.2%

In vitro and animal experimental studies by Broxmeyer's group, authoritative research on umbilical cord blood stem cell biology, showed that umbilical cord blood hematopoietic stem cells that have been frozen for more than 15 years can still maintain their original potential for in vitro proliferation, differentiation, amplification and in vivo implantation.

11-year-old Hunan girl Xiaoying was diagnosed with acute myeloid leukemia in Hunan in December 2014. In order to carry out treatment more effectively and in a timely manner, Xiaoying and her family listened to the advice of local doctors and transferred to Guangzhou Southern Hospital, hoping to undergo umbilical cord blood hematopoietic stem cell transplantation at Southern Hospital. The local umbilical blood bank quickly found a umbilical cord blood that matched 6/6. Unexpectedly, the umbilical blood implanted in her body was collected at the Guangdong Maternal and Child Health Hospital in 2000. It was frozen for 15 years. By age, this umbilical cord blood is actually her "sister". This umbilical cord blood has refreshed the clinical application record of the longest frozen umbilical cord blood bank in the local umbilical cord blood bank. Although the "Miss" has been sleeping for 15 years, it is still vigorous - the pre-transplant resuscitation activity test is as high as 94.2%.

"Sleeping for 15 years, "Mystery Blood Sister" saved 11-year-old leukemia sister"

"Sleeping for 11 years, Umbilical Cord Blood Rescue Wuhan Mom, Frozen in a Can of minus 196 degrees"

In 2012, Beijing Daopei Hospital treated a 49-year-old leukemia patient with a weight of 87 kg. He used a stem cell that had been frozen for 14 years in 1998, and the cell activity reached 96% after resuscitation.

In 2017, a stem cell collected in May 1998 and frozen for 18 years and 11 months was used in Beijing. The cell activity after resuscitation was 97.16%. Among the more than 1,000 cases of outbound warehouses, 58 cases have had frozen for more than 10 years, with an average cell resuscitation activity of about 90%, which fully meets the needs of clinical application. One of them was frozen and the resuscitation activity still reached 94.2% in 15 years. In 2015, an 11-year-old girl with acute myeloid leukemia in Guangdong Province was treated. She is currently in good health.

Professor Broxmeyer's team from Indiana University, an authoritative research on umbilical cord blood stem cell biology in the United States, published in 2011 by the journal BLOOD in the United States, proved that stem cells frozen for 23.5 years can still maintain their original potential for in vitro proliferation, differentiation, amplification and in vivo implantation.

frozen cells is a seemingly simple but highly technical system project. Before the umbilical cord blood is placed in liquid nitrogen for formal storage, in order to prevent stem cells from breaking in a low-temperature environment, a cryoprotective agent is needed to be added to cool down. During the cooling process, professional computer programs should be used to gradually and slowly reduce the temperature until -90℃ before being transferred to low-temperature liquid nitrogen in -196℃ for long-term freezing, so as to ensure and not affect the activity of stem cells.

3. When is the highest quality of frozen cells?

human immunity reaches its peak around the age of 20. As we grow older, the activity of immune cells becomes lower and lower, and what follows is the incidence of disease increases year by year.

Therefore, healthy immune cells are stored in a deep low temperature liquid nitrogen tank at -196°C in advance. When needed, they can be used and taken immediately for convenience and peace of mind. Today's cells are always younger than tomorrow's, and the best storage time is in the present!

storage cells Storage health

With the development of modern medicine, scientists have continuously explored and studied cutting-edge cell technologies. Cell technology has become a powerful guarantee for human life and health, and has played an important role in the fields of health management and disease treatment.

stores healthy and vibrant immune cells and becomes an important means for more and more people to protect their future life. stores immune cells to invest in a healthy wealth for themselves!

In biological cytology research, cancer cells are sometimes cultured and analyzed in vitro.To keep cells well, you must use serum, such as Ausbian® special imported fetal bovine serum , its endotoxin is less than 3Eu/ml, making the cells healthier and making the experiments smoother for customers.

article comes from: Baku Comprehensive Cell Library

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