I believe that friends have all experienced nucleic acid testing for , and many of them have been "manager chiefs". The liquid in virus sampling tube is pink, just like grapefruit juice , which is very cute. What is this pink liquid? If you accidentally get it on your hands, will it be wrong?
What is the red liquid in the virus sampling tube? The red liquid in the
virus sampling tube is called virus preservation solution. At present, the virus sampling tube we use most commonly is the inactivated type. What are the ingredients of
virus nucleic acid preservation solution?
inactivated virus preservation solution mainly includes guanidine salt, bisimidazolidinium, phenol red, buffer , etc. What role do these ingredients in
play? Is it poisonous?
guanidine salt is one of the important components of in the virus preservation solution. guanidine is a very strong monobasic base, and its alkalinity is similar to sodium hydroxide . It can absorb carbon dioxide in the air to form carbonate . Guanidine is unstable under alkaline conditions and is prone to hydrolyzing into ammonia and urea . It is relatively stable under acidic conditions. Therefore, it is generally made into guanidine salts for preservation. Commonly used guanidine salts include guanidine isothiocyanate or guanidine hydrochloride , etc.
guanidine salt is a commonly used protein denaturant, can destroy the protein shell on the surface of the virus, thereby inactive and infectious viruses. Since guanidine salt will eventually hydrolyze into ammonia and urea in aqueous solution, ammonia will be converted into urea under the metabolism of the liver, so the toxicity of guanidine salt can basically be equivalent to urea. And the excess urea is excreted in our body through the kidneys and through the urine.
storage solution also contains a certain amount of biimidazolidin. is usually used as a preservative because it has a certain bactericidal and antibacterial effect. Some of our commonly used facial masks and lotions are also added. There is basically no risk in contact with low doses. If it is stuck on the skin, don’t worry too much. Just wash in water.
Phenol red is an acid-base indicator. It is red in neutral environment, alkaline will turn purple, and acidic will turn yellow. If the storage liquid is contaminated by bacteria (commonly when the storage liquid fails or the sample carries bacteria), the liquid pH drops and it will turn from red to yellow.
In addition, buffer in the virus preservation solution can maintain a relatively stable pH value; other ingredients will ensure that the viral nucleic acid will not lyse in a short period of time.
Therefore, the main function of the virus preservation solution is to inactivate viruses, sterilize and inhibit bacteria, maintain pH and ensure the stability of nucleic acids. If accidentally gets stuck on your hands, don’t worry too much, just rinse it with clean water.
At the same time, I would like to remind everyone that although the liquid in the tube looks pink and cute, cannot touch the eye conjunctiva or the oral and nasal mucosa. If accidentally touches the above parts, please go to the hospital as soon as possible for treatment.
Source: Changsha Disease Control
Editor in charge: Yu Shuai
Supervision and audit: Mu Haoqiang
Forrest Gump recommends reading
From the top 20 reports, see the new picture of people's lives
12306 has a new convenience function!
Just now, the Jiayuguan Cultural Relics Scenic Area was temporarily closed! Recovery time will be notified
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-