Clear zombie cells and return to age 18? Can this brand’s technology story convince you to place an order?
Source | Jumei
Author | Meow Meow
In Silicon Valley , Boston and other places in the United States, there are a group of innovative companies exploring the cutting-edge of beauty technology and synthetic biotechnology. It is one of the innovation centers of beauty and cosmetics in the United States and even the world.
According to a market research report released by Custom Market Insights, the global anti-aging market size will reach US$119.6 billion (about RMB 860 billion) in 2030. With the continuous penetration of the concept of "healthy aging", it is expected that the global anti-aging market size will continue to grow in the future and has huge development potential. If aging is a disease, with the advancement of science and technology, it seems that humans can realize their dream of curing aging and reversing age.
Like China, a large number of anti-aging skin care brands have emerged in the United States, and various companies are competing fiercely in the dimensions of "scientific stories" and "beautiful technology". Today we chose one of the cutting-edge technology-based skin care brands that are "unclear and sharp", in order to bring some different inspiration to the majority of domestic product brands.
The OneSkin company introduced today was born in the famous biotech incubator MBC BioLabs in Silicon Valley, and was founded by four doctors from Brazil. Currently, has successfully raised 7.5 million seed funds, including IndieBio, SOSV, and 2FutureHolding. Well-known investor Peter Diamandis is also one of the board members of OneSkin.
OneSkin's products have not only appeared in major fashion beauty magazines, but also appeared on radio programs such as Dave Asprey, a famous biogeek in Silicon Valley, and Dr. David Sinclair, a famous genetics professor at Harvard University. OneSkin has always emphasized that it is not just a skin care company, but a long life company. They spent 4 years successfully developing OneSkin's first product - Skin Supplement.
From left to right are: Biochemistry and Genomic Bioinformatics Dr. Mariana Boroni, Molecular Biology Dr. Juliana Carvalho, MD, Immunology and Tissue Engineering Dr. Carolina Oliveria Reis, Stem Cell Biology Dr. Alessandra Zonari, MD, Image from OneSkin official website
, OneSkin co-founder, Boroni, developed a biological age calculator called Molclock in 2016 (https://www.molclock.com/ means cell clock) to identify age-related epigenetic changes in skin cells to judge cell age.
Epigenetic refers to the genetic changes in the expression of gene due to environmental factors without changing the DNA sequence. These changes are closely related to people's lifestyles, such as whether to smoke, drink, sun exposure frequency, diet and exercise, living environment, and psychological stress. The data collection of
Molclock also comes from many aspects, including extracting RNA from skin cells and analyzing the expression of up to 20 different genes in the dermis and epidermis; also using skin tissue structure analysis, the cell structure of skin sections was observed under a microscope and compared with the standard age cell structure; even analyzing skin protein production and DNA damage, etc.
Molclock's algorithm training uses data from more than 500 human skin samples and more than 2000 DNA methylation marker. In order to make the data collected by Molclock more comprehensive and objective, OneSkin used a laboratory-made Petri dish simulated skin model (Human Skin Equivalent, HSE) for in vitro testing. The skin cells used for culture (fibroblasts and keratinocytes) are from donors from different races and ages.
is different from other companies that only test newborn skin models. OneSkin not only detects young skin, but also detects other age stages, such as 30-70 years old, so it can detect thousands of skin samples of different types/ages at the same time. It was later confirmed that the laboratory's HSE in vitro test results are consistent with the clinical test results after real-person skin biopsy sampling.
simulated skin model (Human Skin Equivalent), the picture comes from OneSkin official website
simulated skin model (Human Skin Equivalent) and the cell structure of real-person skin slices, the picture comes from OneSkin official website
By using the cell clock Molclock and simulated skin model Human Skin For the two powerful tools of Equivalent, OneSkin has screened more than 1,000 anti-aging peptides, and finally discovered OS-01, a decapeptide that can penetrate the keratin barrier in 2018:
OneSkin's patent decapeptide (OS-01) structure. The picture comes from OneSkin's official website
OneSkin's patent decapeptic peptide (OS-01). After multiple clinical trials, it has proved that in addition to the common effects of increasing skin elasticity, stimulating the generation of collagen , wrinkle removal and moisturizing, the most unique thing is that it can reduce the "biological age" of the skin by reducing the accumulation of senescent cells (commonly known as "zombie cells" Senescent Cells), and can reduce senescent cells by as much as 25-50%. Make the skin not only look younger, but repair cells, especially damage caused by ultraviolet rays, which can truly make the skin younger and extend the skin's life cycle .
Scientists stained with β-galactosidase (Beta-Galactosidase)
The right picture shows the number of senescent cells that have been used with OS-01 has been significantly reduced. The picture comes from OneSkin official website
The comparison of the cell structure of the 55-year-old skin before and after use. The right picture shows the skin that has been used with OS-01 has become significantly thicker. The picture comes from OneSkin official website
The so-called "zombie cells" are that cells will enter the aging phase before stopping division. When aging starts, the key factors CDK-1 and Lamin The concentration of B1 (cell cycle-dependent protein kinase-1 and laminin) decreases. At this time, the cells do not die or divide and reproduce, spreading aging mediators (Cytokines) in the body like zombies, causing the surrounding healthy cells to start to get inflamed and even lead to stem cell failure. Usually in healthy young people, the immune system can remove these "zombie cells" in time, but as they get older, the cleaning becomes slower, and the "zombie cells" will gradually accumulate.
Schematic diagram of normal cells transforming into senescent cells, picture from National Institutes of Health
Many cell biologists believe that although a few "zombie cells" can heal wounds or prevent cancer cells from spreading, the accumulation of "zombie cells" as they age may be the culprit of aging. The accumulation of "zombie cells" is also related to many diseases, such as diabetes , arthritis, cardiovascular diseases, and even cancer.
Compared with other organs and skin in the body, the largest organ in the human body, this human body is more likely to be exposed to external environmental pressure, such as ultraviolet rays, air pollution, cold, etc. After cell aging is initiated, MMP-1 will also increase and activate MMP-1 ( matrix metalloproteinase -1), and MMP-1 will decompose elastin and collagen, causing collagen breakage and damage to the skin barrier, making wrinkles deeper and skin elasticity worse.
Dr. James Kirkland from Mayo Hospital and his team have conducted experiments to transfer the extracted "zombie cells" to young mice. These cells spread rapidly, contaminating healthy cells, causing inflammation and even failure of the mice's organs. But after removing the "zombie cells", the mice regained their vitality and became younger, and even improved hair density and kidney function. Jennifer Elisseeff, a medical engineer from Johns Hopkins University, also found that removing senescent cells in mice alone can stimulate the regeneration of damaged cartilage tissue.
Two twin mice, the mouse on the right looks younger after being removed from senescent cells. The picture comes from Mayo Hospital
At the same time, a research paper published by OneSkin stated that data from multiple clinical experimental data confirmed that OS-01 is superior to traditional A alcohol/A acid in skin anti-aging, promoting collagen production, hyaluronic acid generation, and barrier repair. Traditional A alcohol/A acid uses stimulating cell renewal to promote epidermal shedding and achieves anti-aging effects. However, for dry or sensitive skin, A alcohol/A acid is usually too irritating. If used improperly, it may even cause long-term damage to the skin, such as thinning of the skin.OS-01 can improve cell repair capabilities and clean up senescent cells without irritating the skin.
Gene expression analysis results after treatment with OS-01 and A alcohol can be seen that OS-01 is better than A alcohol in reducing aging and inflammation. The production results of collagen and hyaluronic acid are also comparable to A alcohol. The picture comes from OneSkin official website
The mechanism of action of OS-01 is similar to applying rapamycin (RAPA, a clinically used immunosuppressant that can clear senescent cells) on the skin surface, but will not produce similar side effects. Although it cannot directly damage senescent cells, it reduces the production and activity of senescent cells, which allows the human immune system to have more time to clear senescent cells in time.
OS-01 is mainly targeting the inflammatory factors IL-6, IL-8 and MMP-1 in the aging-related secretory phenotype (SASP). OS-01 regulates the pathways that control oxidative stress and inflammation by binding to and interacting with the cytoplasm in the cell membrane. In the test results of the 3D skin model and biopsy samples, OS-01 enhances the repair mechanism of DNA and downregulates the signaling pathway associated with aging, thereby reducing the production and accumulation of senescent cells.
OneSkin also conducted a 12-week double-blind clinical study consisting of 22 female participants aged 47 to 65 years. Participants applied the essence containing no OS-01 on their right face twice a day for 12 weeks and the essence containing OS-01 on their left face. The survey results show that:
The skin barrier on the left face of all users increased by 15% on average
90% of users said that the skin elasticity increased
95.5% of users said that the skin uniformity was increased, and the gloss, pores and firmness were improved
87% of users said that the fine lines and wrinkles were reduced
100% of users said that the overall appearance and smoothness of the skin were improved
Many users on Reddit said that they could feel obvious changes after 3-6 months of use. OneSkin Serum also reduces facial acne and allows acne to heal faster, indicating that the product is suitable for all skin types.
Speaking of OneSkin's emphasis on penetrating the stratum corneum, although the molecular weight of OS-01 decapeptide is 1182 daltons, in theory, molecules with a ratio of more than 500 daltons are difficult to penetrate the stratum corneum barrier, but the ability of molecules to penetrate the stratum corneum not only depends on the size of the molecule, but also includes the polarity of the molecule and the solubility of the . OS-01 has a slight positive charge, coupled with the formula itself, the emulsification system simulates the extracellular lipids of the stratum corneum, thereby promoting penetration.
OneSkin Essence Milk (OS-01 FACE Topical Supplement) product display, the picture comes from Jumei reporter
emulsion texture display, the picture comes from Jumei reporter
This product has no fragrance and pigmentation, OS-01 has also undergone a number of skin allergy tests (RIPT) and toxicity tests, and no adverse reactions were found, and no pore blockage or allergic reactions were found after the reporter used it. In addition to containing the patented OS-01 decapeptide, the essence milk also contains the following active ingredient :
Calycophyllum Spruceanum Bark Oleic Extract (Pau Mulato) Spruce Light Bark Tree Bark Extract: Antibacterial and Anti-inflammatory and Antioxidant.
Carapa Guianensis Seed (Andiroba) Oil Acid Slag Tree Oil: promotes collagen synthesis, anti-inflammatory, and promotes cell renewal and repair.
Pentaclethra Macroloba (Pracaxi) Oil Bacacas Fruit Oil: promotes wound healing, antioxidant, anti-inflammatory, and anti-stress.
Niacinamide Niacinamide : whitening, oil control, anti-aging, repair the stratum corneal lipid barrier, and improve skin resistance .
- hyaluronic acid of different molecular sizes: macromolecules can form films to protect and moisturize and isolate pollution, while small molecules can repair, antioxidant, anti-inflammatory and anti-aging.
Jumei The reporter personally tried OS-01 essence milk (FACE Topical Supplement). After using it for a month, the reporter could clearly observe that the skin's gloss increased with the naked eye, the skin texture was more delicate and no longer easy to dry. The redness on both cheeks was significantly reduced.
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In today's tide of efficacy skin care, the focus of competition among domestic products often falls on innovative raw materials and efficacy verification, but its basic mechanism, effectiveness logic, skin model verification process, etc. are often vague. This leads to the lack of evidence links in the scientific story content of the brand, and it is impossible to strongly convince deep-seated ingredient parties such as professional users and vertical KOLs, leaving hidden dangers for the further spread of the brand.
The OneSkin brand selected in this article has made unique technological innovations in at least the following aspects and prepared clear and evidence-based scientific stories, including at least the following aspects:
Anti-aging pathway selection: Clean up "zombie cells"
Efficacy test: Simulated skin model in vitro test In vitro test Simulated skin model
evaluation model: Molclock's biological age calculator
Patent raw materials: decapeptide (OS-01) efficacy logic and evidence
Through the above complete content framework, in just a few thousand words, we can understand the technical basis and unique effects of the brand. Hope the case in this article will be helpful to you.
The author of this article Crystal Chen studied for undergraduate food science at at . Later, he studied for a PhD in pharmacy at at at . He has served as a formulator and product development position in several functional skin care products and nanotechnology companies in the United States. As a special correspondent in Jumei in the United States, she will lead us into the United States' cutting-edge beauty and cosmetic technology companies and innovation projects, bringing the latest technological information about American peers.
Reference materials for this article:
1. Mesenchymal Stem Cell Senescence and Rejuvenation: Current Status and Challenges ,frontiers
2. The role of senescent cells in ageing ,PMC PubMed Central®
3. Senescence and aging: Causes, consequences, and therapeutic avenues ,PMC PubMed Central®
4. Senolytics in idiopathic pulmonary fibrosis: Results from a first-in-human, open-label, pilot .study ,eBioMeficine
5. In vitro and in vivo toxicity assessment of the senotherapeutic Peptide 14 ,ScienceDirect thirome analysis ,BMC
7. Calycophyllum spruceanum (Benth.), the Amazonian “Tree of Youth” Prolongs Longevity and Enhances Stress Resistance in Caenorhabditis elegans ,ResearchGate
8. Does cellular sense hold secrets for healthier aging? ,National Institute on Aging
9. Human skin equivalents: Impaired barrier function in relation to the lipid and protein properties of the stratum corner ,ScienceDirectt
10. OneSkin’s 12-Week Clinical Study To Validate The Effects Of OS-01 FACE ,OneSkin
This article is the second article in this series, which will take you to learn about the latest new technology for therapeutic peptide skin care in Silicon Valley. The previous link is as follows:
Experience the latest beauty technology in Silicon Valley: eyelash grafting robot
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