Have you ever tested nucleic acid
Have you ever been a "pilot leader"?
. I wonder if you are curious about the liquid in the nucleic acid sampling tube. What exactly does
work? Why is
red? What is the red liquid in the nucleic acid tube
? What is the function of
?
The red liquid in the nucleic acid sampling tube is called the viral nucleic acid storage solution .
guanidine salt is one of the important components of viral nucleic acid preservation solution. Guanidine is a very strong monobasic base, and its alkalinity is similar to that of sodium hydroxide . It can absorb carbon dioxide in the air to form carbonate . Guanidine is unstable under alkaline conditions and is prone to hydrolyzing into ammonia and urea , which is relatively stable under acidic conditions. Therefore, it is generally made into guanidine salts for preservation. Commonly used guanidine salts include guanidine isothiocyanate or guanidine hydrochloride , etc.
Guanidine salt is a commonly used protein denaturant that can destroy the protein shell on the surface of the virus, thereby exposing the nucleic acid to be tested. Since guanidine salt will eventually hydrolyze into ammonia and urea in aqueous solution, and ammonia will be converted into urea under the metabolism of the liver, the toxicity of guanidine salt can basically be equivalent to urea. And the excess urea is excreted in our body through the kidneys and through the urine.
storage solution also contains a certain amount of biimidazolidine . Because it has a certain bactericidal and antibacterial effect, it is usually used as a preservative. Some of the commonly used facial masks and lotions are also added. There is basically no risk in contact with at low doses. If it is stuck on the skin, don’t worry too much. Just wash it in clean water.
As for why the storage solution is red?
is because a reagent called phenol red is added to it
phenol red is an acid-base indicator. is red in neutral environment, alkaline will turn purple, and acidic will turn yellow. If the storage liquid is contaminated by bacteria (commonly when the storage liquid fails or the sample carries bacteria), the liquid pH drops and it will turn from red to yellow.
In addition, the inorganic salts in the nucleic acid storage solution can maintain a relatively stable pH value; the nutrients in it will ensure that the viral nucleic acid will not cleave in a short period of time.
Speaking of this, we can basically understand that the main function of
"red water"
is
inactivate virus
bactericidal and antibacterial
and
ensure the stability of nucleic acid
In short, the ultimate purpose of nucleic acid preservation solution is to allow nucleic acids in the test sample to be properly preserved and viruses are discovered in a timely manner.
In addition, nucleic acid detection of liquid is intentional, is for the purpose of giving eye-catching hints and distinctions. is also OK if it is a colorless and transparent liquid, but the red is more eye-catching.
What if
someone accidentally poured nucleic acid detection solution
that looks like raspberry juice into his mouth?
If you accidentally eat it, you should go to a professional medical institution for treatment in time, because the ingredients in the nucleic acid testing solution are buffer and some chemical components. If you find that you accidentally eat it, you must go to the hospital to deal with it as soon as possible. How to ensure the transportation safety of nucleic acid tubes in
? Where will
end up going? After
is completed, the nucleic acid sample storage tube will be immediately loaded into a sealed bag, then loaded into a yellow medical bag, and finally loaded into , a biosafety transport box for . There is an ice pack inside the box to ensure that the test sample is in a low temperature of 4 degrees Celsius. Because the lower the temperature of , the more stable the nucleic acid is. With the two "double insurances" of "low temperature protection" and "nucleic acid preservation solution", the accuracy of laboratory detection results can be well guaranteed.
For the transfer time, according to the national unified standards, samples are collected every 2 hours for transportation, and ensures that the samples are delivered to the laboratory within three hours after the sample is collected and tested by . After the nucleic acid test is completed, it is sealed and disinfected, and sent to the terminal treatment plant by a special person and a special vehicle. The transportation process is safe and pollution-free. Generally, the tubes or specimens of negative nucleic acid storage solution are centrally cleaned and processed by professional companies according to medical waste treatment standards.Positive specimens need to be autoclaved and then treated according to general medical waste.
After reading the article, I believe that you
have a further understanding of "red liquid"
In the future, when the "pipe is long", you should be careful to protect the nucleic acid tube
. It will also save you some attention when you are busy with "big whites"!
, it requires the power of your "arm".
Collection of pictures and texts: Reprinted from Rongxian County, Overseas Chinese hometown,
Editor: Ma Yongping Reviewed: Shuimu Feige
Produced by: Fumian District Media Center
Submission Email: fumianxuanchuan@126.com
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